Other Species Collections
Zebrafish Gene Collection Project Summary
At the April 2002 meeting of zebrafish researchers at the NIH, a full-length cDNA
project was considered the top priority for NIH funding. Consequently, the
Zebrafish Gene Collection (ZGC) was launched in August 2002, as a subproject of the existing
Mammalian Gene Collection project. The general approach is to acquire or construct
high-quality cDNA libraries, screen the libraries by sequencing the 5' end of clones,
identify putative full open reading frame clones, and then carry out full-length sequencing of
the selected clones.
The ZGC will modify the MGC's procedures slightly. Instead of selecting clones on a continuous basis, the ZGC will be a phased project:
- Initially, the ZGC will collect 5' sequences from clones in various libraries over a 3-month period.
- At the end of that time, an external panel of scientists will review the list of all the putative full-length clones eligible for full-length sequencing and prioritize those for sequencing.
- This process will then be repeated quarterly.
- In addition, the ZGC will collaborate with other researchers who have significant existing collections of putative full-length clones to sequence those clones.
As part of the zebrafish genome project, a collection of full-length cDNAs has many potential applications, such as:
- Enumerating the genes, improving the accuracy of gene identification, and annotating other genomes.
- Allowing genetic manipulations such as complementation tests or over expression analysis.
- Providing information about the 5' untranslated or AUG regions of genes for construction of morpholinos, the primary tool for gene expression "knockdown" in zebrafish.
- Providing an invaluable reagent for any researcher studying a particular gene or developing arrays or other tools to study multiple genes.
All materials produced by the ZGC, including libraries, ESTs, and full-length clones are publicly