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pOTB7 Library Method Overview

A set of thirty-six MGC libraries was made in this vector by Dr. Gerry Rubin, University of California, Berkeley CA. Briefly, the method was as follows:
  • Poly A+RNA was reverse trancribed using an anchored oligo-dT primer with an Xho I site.
  • Double stranded cDNA was prepared using a cDNA synthesis kit.
  • The double stranded cDNA was ligated to Eco RI adaptors, digested with Xho I, size selected over a column, and ligated into the vector.
  • The pOTB7 vector was constructed by Dr. Michael Brownstein, National Institute of Mental Health, NIH, Bethesda, MD.