Other Species Collections
pOTB7 Library Method Overview
A set of thirty-six MGC libraries was made in this vector
by Dr. Gerry Rubin, University of California, Berkeley CA. Briefly,
the method was as follows:
- Poly A+RNA was reverse trancribed using an anchored oligo-dT
primer with an Xho I site.
- Double stranded cDNA was prepared using a cDNA synthesis kit.
- The double stranded cDNA was ligated to Eco RI adaptors, digested with
Xho I, size selected over a column, and ligated into the vector.
- The pOTB7 vector was constructed by Dr. Michael Brownstein, National Institute of Mental Health, NIH, Bethesda, MD.