Other Species Collections
pCMV-SPORT6 Library Method Overview
Prior to January 2001, Life Technologies, Invitrogen Corp., Carlsbad, CA, constructed non-normalized (standard) libraries
in the pCMV-SPORT6 vector.
Briefly, the library method was as follows:
- First strand cDNA was synthesized from polyA+mRNA using Invitrogen Superscript II RT and an oligo-dT primer
containing a Not I site.
- Second strand cDNA was synthesized.
- Sal I adapters were ligated onto the double stranded
cDNA which was then digested with Not I
creating 5'-Sal I/3'- Not I inserts.
- This cDNA was size-fractionated on a column and ligated
into the pCMV-Sport6 vector.